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KMID : 0903519970400020163
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1997 Volume.40 No. 2 p.163 ~ p.166
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Preparation of Sophorose
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김신일/Kim, Shin Il
박종대/이유희/백남인/이미자/김해영/PARK, Jong Dae/Lee, You Hui/Baek, Nam In/Lee, Mi Ja/Kim, Hae Yeong
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Abstract
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A yeast, Torulopsis bombicola, was cultured in the media fortified with soybean oil as a additional carbon source for 7 days with reciprocal shaking. From the culture broth, sophorose-lipid was isolated and treated with alkali to afford sophorose. The sophorose contained in the medium was acetylated and isolated through silica gel column chromatography. The aceylated sophorose was hydrolyzed with 5% KOH at room temperature to give rise to sophorose. Meanwhile, the MeOH extracts obtained from the pod of Sophora japonica was solvent-fractionated with n-BuOH and H₂O, and butanolic layer was chromatographed on silica gel column to afford a flavonoide-glycoside. The glycoside was hydrolyzed with 0.02 N H₂SO₄, to yield sophorose.
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KEYWORD
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